KMID : 0620920120440090529
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Experimental & Molecular Medicine 2012 Volume.44 No. 9 p.529 ~ p.535
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Over-expression of extracellular superoxide dismutase in mouse synovial tissue attenuates the inflammatory arthritis
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Yu Dong-Hoon
Yi Jun-Koo Yuh Hyung-Soo Park Seo-jin Kim Hei-Jung Bae Ki-Beom Ji Young-Rae Kim Na-Ri Park Si-Jun Kim Do-Hyung Kim Sung-Hyun Kim Myoung-Ok Lee Jeong-Woong Ryoo Zae-Young
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Abstract
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Oxidative stress such as reactive oxygen species (ROS) within the inflamed joint have been indicated as being involved as inflammatory mediators in the induction of arthritis. Correlations between extracellular-superoxide dismutase (EC-SOD) and inflammatory arthritis have been shown in several animal models of RA. However, there is a question whether the over-expression of EC-SOD on arthritic joint also could suppress the progression of disease or not. In the present study, the effect on the synovial tissue of experimental arthritis was investigated using EC-SOD over-expressing transgenic mice. The over-expression of EC-SOD in joint tissue was confirmed by RT-PCR and immunohistochemistry. The degree of the inflammation in EC-SOD transgenic mice was suppressed in the collagen-induced arthritis model. In a cytokine assay, the production of pro-inflammatory cytokines such as, IL-1¥â, TNF¥á, and matrix metalloproteinases (MMPs) was decreased in fibroblast-like synoviocyte (FLS) but not in peripheral blood. Histological examination also showed repressed cartilage destruction and bone in EC-SOD transgenic mice. In conclusion, these data suggest that the over-expression of EC-SOD in FLS contributes to the activation of FLS and protection from joint destruction by depressing the production of the pro-inflammatory cytokines and MMPs. These results provide EC-SOD transgenic mice with a useful animal model for inflammatory arthritis research.
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KEYWORD
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arthritis, experimental, reactive oxygen species, rheumatoid arthritis, superoxide dismutase, synovial membrane
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